【摘要】 目的探讨血管紧张素转换酶 (angiotensinconvertingenzyme ,ACE)基因插入 /缺失 (insertion/deletion ,I/D)多态性与冠心病血瘀证的关系。方法用PCR法检测 4 8例血瘀证和 5 2例非血瘀证冠心病患者及 5 4名健康人的ACE基因型 ,同时检测内皮素 (endothelins ,ET)、血管紧张素Ⅱ (angiotensinⅡ ,AgⅡ )、一氧化氮 (nitrogenmonoxide ,NO)值。 结果冠心病血瘀证组ACEDD基因型及D等位基因频率高于非血瘀证组和健康对照组 (P <0 0 1)。ET/NO冠心病血瘀证组明显升高 ,与健康对照组比较差异有显著性(P <0 0 1)。ET、AgⅡ冠心病血瘀证组明显高于非血瘀证组和健康对照组 (P <0 0 5 ,P <0 0 1)。ET/NO、AgⅡ各组DD型均高于II型和ID型 ,其中以冠心病血瘀证组DD型为最高 ,与其他两组比较AgⅡ差异有显著性 (P <0 0 5 ,P <0 0 1) ,与健康对照组比较ET/NO差异有显著性 (P <0 0 1)。结论DD型ACE基因可能为冠心病血瘀证发病的易感基因更多还原

【Abstract】 Objective To explore the relationship between the insertion/deletion (I/D) polymorphism of angiotensin converting enzyme (ACE), and blood stasis syndrome (BSS) in patients with coronary heart disease (CHD). Methods The ACE gene type in 48 patients of CHD of BSS type, 52 CHD patients of non BSS type and 54 healthy subjects (control) was determined by PCR assay, also levels of endothelin (ET), angiotensin Ⅱ (AgⅡ), and nitric oxide (NO) were determined. Results Occurrence of DD genotype and allele genotype of ACE gene was higher in patients of BSS than that in patients of non BSS and control (P<0 01). ET/NO level was higher in patients of BSS than that in control (P<0 01). ET and AgⅡ levels in patients of BSS were significantly higher than those in patients of non BSS (P<0 05) and control (P<0 01). Levels of ET/NO and AgⅡ in subjects with DD genotype in various groups were higher than those in subjects with AgⅡ or ID genotype, the highest level occurred in patients of BSS with DD genotype, when compared with the other two groups, the difference in AgⅡ was significant (P<0 05 and P<0 01), when compared with control, the difference in ET/NO was significant (P<0 01). Conclusion DD genotype of ACE gene may be the susceptible gene of CHD in patients of BSS type.更多还原