【摘要】 目的 探讨动脉粥样硬化模型大鼠随着造模时间的推移“痰瘀”演变规律。方法 采用高脂饲料喂饲法复制大鼠动脉粥样硬化模型,用生化法及免疫组化方法动态检测血脂、血液流变学、血糖、胰岛素及血管平滑肌细胞(VSMC)肌动蛋白表达的变化。结果 随着动脉粥样硬化大鼠由痰到瘀症状的变化(即血脂、血液流变学、血糖、胰岛素及胰岛素敏感指数等指标均随病情加重而变化),VSMC肌动蛋白表达逐渐增加。结论 VSMC肌动蛋白表达是动脉粥样硬化大鼠由痰到瘀的分子机制。更多还原

【Abstract】 To explore the dynamic change of Phlegm-stasis in the rat atherosclerotic model as the time goes on. Methods Adopting high fat forage fed to develop the atherosclerotic model in rats, and the changes of blood lipid, hemorrheology, blood glucose, insulin and vascular smooth muscle cell (VSMC) actin expression were detected by biochemical and immunohistochemical assay at various time points after modeling. Results The expression of VSMC actin gradually increased along with the change of model rats’ Syndrome from Phlegm to stasis, i.e. , the change of parameters, including blood lipid, hemorrheologic parameters, blood glucose, insulin and insulin sensitive indexes along with the aggravation of disease. Conclusion The expression of VSMC actin could be the molecular mechanism for the Syndrome developing from Phlegm to stasis in atherosclerotic rats.更多还原