【摘要】 目的　本院 1996～ 2 0 0 3年诊断明确的 175例恶性血液病 (包括急性白血病和淋巴瘤 )进行IgH和TCRγ基因重排实验 ,以探求恶性血液病与基因重排的相关性。方法　用聚合酶链式反应方法检测 175例恶性血液病骨髓或外周血单个核细胞(MNC)中单克隆性免疫球蛋白IgH和T细胞受体TCRγ基因重排。结果　 4 3例ALL有 2 1例发生IgH重排 ,18例发生TCRγ重排 ,4例IgH和TCRγ都发生重排。 2 7例AML有 10例发生IgH重排 ,13例发生TCRγ重排 ,4例IgH和TCRγ都发生重排。 10 5例淋巴瘤患者中 5 7例发生IgH重排 ,2 9例发生TCRγ重排 ,19例IgH和TCRγ都发生重排。结论　人类恶性淋巴增殖性疾病 (急慢性淋巴细胞白血病和恶性淋巴瘤 )是阻断在不同分化阶段的恶性淋巴细胞的克隆性增殖。IgH和TCRγ基因重排的检测为B、T淋巴增殖性疾病提供了克隆标记。但在非淋巴性恶性增殖疾病如ANLL中确实存在系列不保真现象。不仅具有较高的IgH重排 ,而且还具有较高的TCRγ基因重排更多还原

【Abstract】 Objective To explore the clinical significance of rearrangements of immunoglobulin heavy chain (IgH) and T cell receptor(TCR) γ genes in patients with hemopoietic malignancies. Methods Polymerase chain reaction (PCR) was used to study IgH and TCRγ genes rearrangements in 175 hemopoietic malignancies patients. Results The gene rearrangement of IgH accounted for 50.3%, TCR γ 65 cases for 34.2%, both positive 27 cases (15%), The gene rearrangement rate was as high as 85.1%. Conclusion This method is sensitive to hemopoietic malignancies. Lineage infidelity of immunoglobulin gene rearrangements dose exists in ANLL(acute nonlymphocytic leukemia).更多还原