【摘要】 目的　应用RT PCR法检测乳腺癌患者外周血中的humanmammaglobin(hMAM )mRNA和CEAmRNA表达情况。 方法　用RT PCR法 ,检测 5 2例乳腺癌患者外周血中hMAMmRNA和CEAmRNA的表达情况 ,另抽取 2 8例乳腺良性疾病患者和 10例健康志愿者外周血标本作为对照。结果　 5 2例乳腺癌患者外周血中hMAMmRNA和CEAmRNA的阳性率分别为 30 .8%、34.6 %。 2 8例乳腺良性疾病患者和 10例健康志愿者外周血中hMAMmRNA、CEAmRNA表达均为阴性。两者的阳性表达均与患者的腋淋巴结状况、TNM分期差异有显著性意义 (P <0 .0 5 )。而与患者的年龄、原发肿瘤大小、病理类型以及激素受体状况等均无显著性差异 (P >0 .0 5 )。结论　hMAMmRNA、CEAmRNA均可作为标志物来检测乳腺患者外周血中微转移 ,联合检测可增加外周血中循环肿瘤细胞的检测率。更多还原

【Abstract】 Objective Detection of breast cancer micrometastasis in peripheral blood by using human mammaglobin(hMAM) mRNA and CEA mRNA RT PCR. Methods Expressions of hMAM mRNA and CEA mRNA in peripheral blood were detected in 52 cases of breast cancer, and 28 patients with breast benign lesions and 10 heathly volunteers as controls by RT PCR. Results The positive hMAM mRNA and CEA mRNA expession were 30.8% and 34.6%, No positive hMAM mRNA and CEA mRNA were found in peripheral blood of 28 patients with breast benign lesions and 10 heathly volunteers. The hMAM mRNA and CEA mRNA expessions were correlated with axillary lymph nodes status and TNM stage. There was no significant difference between micrometastasis and any of other clinical parameters. Conclusion The hMAM mRNA and CEA mRNA may be potential tumor markers for detecting micrometastasis and recurrence in peripheral blood.更多还原