【摘要】 目的　建立可研究肿瘤微血管的分子学特性及结构特性的细胞模型 ,应用于药物筛选及靶向药物输送的研究。方法　通过TRANSWELL共培养肿瘤细胞MCF 7和内皮细胞HUVEC的方法 ,建立肿瘤微血管细胞模型 ,检测了该模型下的细胞形态、结构、及通透性的变化。结果　在肿瘤细胞共生条件下的内皮细胞层结构疏松 ,微环境呈明显酸性 ,而且细胞间隙增大 ,对 10 0纳米左右粒子的通透性有显著提高。结论　这一细胞模型的建立将有助于肿瘤血管的分子、结构特性以及相关药物的筛选和输送研究。更多还原

【Abstract】 Objective To study the molecular, morphological and physiological features of tumor microvasculature, we developed a TRANSWELL co culture system containing human umbilical vein endothelial cells(HUVECs) and MCF 7 human mammary cancer cells. In the presence of co cultured tumor cells, there were marked changes of the HUVEC endothelium morphology. We can detect an increased permeability of 100nm particles through the layer. The model can be used to study the molecular detail of the tumor microvasculature, as well as provide a more physiological relevant cell model to screen for tumor targeting ligands and delivery systems.更多还原