【摘要】 目的 :观察还原型谷胱甘肽 (GSH)合中药抗震止痉胶囊对帕金森病 (PD )大鼠模型的异常形为、黑质抗氧化系统及细胞形态学的影响 ,并探讨其作用机制。方法 :应用 6-羟基多巴胺立体定向注射制作PD大鼠模型。将2 4只大鼠随机分为 3组 (每组 8只 ) :模型组、治疗组 (GSH合抗震止痉胶囊组 )、假手术组 ,分别给予相应处理 ,共 45d ,给药前后均进行行为学测试 ,给药结束后每组随机选取 2只大鼠 ,分别行免疫组化和电镜观察 ,测定其余大鼠黑质区GSH -Px、MDA及活性氧水平。结果 :①治疗组可明显改善大鼠旋转行为 (P <0 .0 5 ) ;②治疗组能减轻黑质区氧化应激损伤 ;③免疫组化发现治疗组TH -IR神经元较模型组明显增多 (P <0 .0 0 1) ;④电镜下发现治疗组可部分延缓凋亡进程。结论 :GSH合抗震止痉胶囊能部分改善大鼠旋转行为 ,减轻PD模型大鼠黑质区氧化应激损伤 ,并对残存DA能神经元具有一定保护作用。更多还原

【Abstract】 Objective:To observe the effects of Glutathione Combined with Kang-zhen Zhijing Capsule on the abnormal behavior, nigral antioxidation system and cell morphology of PD model rat and study its mechanism. Methods:The model rats for PD were established through stereotoxic microinjection of 6-hydroxydopamine (6-OHDA). The 24 model rats after behavior test were randomly divided into 3 groups: Model group(MG), Glutathione Combined with Kang-zhen Zhijing Capsule group (GKG) and sham-operated group (SG). Every group was given relevant treatment for 45 days. All rats behavior were tested before and after giving medicine. 2 rats randomly chosen from every group were observed under immunohistochemistry and electron-microscope. The activity of GSH-Px, content of MDA and ROS of nigra of all other rats were assayed. Results:①GSH Combined with Kang-zhen Zhijing Capsule had obvious effect on its rotation behavior; ②Glutathione Combined with Kang-zhen Zhijing Capsule could decrease the nigral oxidative stress of PD model rats; ③The TH-IR neurons of damaged side of GG increased compared with MG (all P<0.001); ④GSH could partly postpone the course of apoptosis. Conclusion:GSH Combined with Kang-zhen Zhijing Capsule could ameliorate partially PD model rats rotation behavior, decrease the nigral oxidative stress of PD model rats, and protect surviving dopaminergic neurons.更多还原