【摘要】 目的　从细胞水平研究异紫堇啡碱对PGF2α诱导心肌肥大的作用。方法　利用培养的新生大鼠心肌细胞 ,以细胞直径大小和蛋白含量为心肌肥大反应的指标 ,用Fura 2 /AM为荧光指示剂测定心肌细胞内钙浓度。结果　异紫堇啡碱 10 4mol/L可明显抑制PGF2α诱导的心肌细胞肥大和细胞内钙增加。结论　异紫堇啡碱可抑制PGF2α诱导心肌细胞肥大 ,该作用可能与其抑制细胞内钙增加有关更多还原

【Abstract】 Objective To observe the effects of isocorydine on cardiac myocyte hypertrophy induced by prostaglandin F2α. Methods Neonatal rat cardiomyocyte hypertrophic response was assayed by measuring cell diameter and protein content. Using Fura 2/AM as a fluorescent indicator,the intracellular free calcium concentration ( [Ca 2+ ]i ) was measured. Results Isocorydine at 10 -4 mol/L could inhibit cardiomyocyte hypertrophy and the increase of [Ca 2+ ]i induced by prostaglandin F2α. Conclusion Cardiomyocyte hypertrophyic response induced by prostaglandin F2α could be abolished by isocorydine partly, which may be related to its inhibitory effects on the increase of [Ca2+]i.更多还原