【摘要】 本文综述了用分光光度法,荧光检测法及高效液相色谱法测定藻类叶绿素及其降解产物的方法.分别详细介绍了各种方法的设备,程序,计算公式及特点.此外,还介绍了藻类类胡萝卜素的高效液相色谱测定方法.本文按测定方法分为六个部分:(1)叶绿素a,b和c的分光光度法测定(三色法);(2)叶绿素a的荧光检测法;(3)存在脱镁叶绿素a时叶绿素a的分光光度法测定;(4)存在脱镁叶绿素a时叶绿素a的荧光法测定;(5)高效液相色谱(HPLC)测定藻类的叶绿素及它们的降解产物;(6)HPLC测定藻类叶绿素及类胡萝卜素.更多还原

【Abstract】 Methods for determination of chlorophylls and their degradation products by spectrophotometry, fluorometry, and high-performance liquid chromatography were summarized in this paper. The equipment, procedure, calculation formula and characteristic of each method were described in detail. In addition, determination of carotenoid pigments by HPLC was introduced. The methods for determination are presented in this paper.更多还原