【摘要】 应用高效液相色谱法研究了黄芪的指纹图谱 ,并对毛蕊异黄酮、芒柄花素及黄芪甲苷进行了含量测定。结果 ,找出了 2 6个共有峰 ,12个样品之间的相似度都在 90 %以上。本样品处理方法简单 ,建立的黄芪指纹图谱稳定性、重复性好。可用于控制黄芪药材的质量。更多还原

【Abstract】 Objective:To study on chromatography fingerprint(CFP) of Huangqi herbs.Method: HPLC was used on a Hypersil ODS column with water-acetonitriles gradient elution.CFP was referenced to formononetin,calycosin and then the contents of astragaloside Ⅳ,formononetin and calycosin was determinted.Result:26 common peaks were pointed out,their similarity of 12 samples were more than 90%.Conclusion:The handling of samples were simple. The study method on chromatography fingerprint of Huangqi were reliable. Good repetition and their similarity accorded with the demand on "the technology guide to experiment studies on chromatography fingerprint of injection of traditional Chinese medicine (TCM)",which was issued by the nation pharmacopoeial commission.The chromatography fingerprint and determination of index composition could be used to comprehensively control the quality of Huangqi.更多还原