【摘要】 以五指毛桃茎节为外植体 ,1/2MS培养基加不同植物生长调节剂的组培实验结果表明 :不定芽最适诱导培养基为 1/2MS +BA1 0mg/L +NAA1 0mg/L和 1/2MS +BA1 5mg/L +NAA0 5mg/L ,增殖培养基为 1/2MS +6 -BA0 5mg/L ,生根培养基为 1/2MS +IBA 1mg/L。本文还对组织培养过程中 ,导致褐变产生的因素及防止褐变的方法进行了探讨更多还原

【Abstract】 The tissue culture of Ficus hirta Vahl.was studied.The nodes were used as explants and 1/2MS media with different plant growth regulators were tested.The result showed that the adventitious bud differentiation medium was 1/2MS+BA1.0mg/L+NAA1.0 mg/L and 1/2MS+BA1.5mg/L+NAA0.5mg/L,the media for multiplication was 1/2MS+6-BA0.5mg/L,and the medium for rooting was 1/2MS+IBA1 mg/L.The cause of nigrescence in the tissue culture and its preventive methods were also discussed in this paper.更多还原