【摘要】 目的 :开发安全有效的抗呼吸道合胞病毒的新药。方法 :采用细胞病变抑制实验观察甘草在Hela细胞中对呼吸道合胞病毒的抑制作用。结果 :甘草半数中毒浓度 (TC50 )为 3 4 3g/L ;抑制呼吸道合胞病毒的半数有效浓度 (EC50 )为 0 2 5 35g/L ,治疗指数 (TI)为 13 5 3;甘草对呼吸道合胞病毒的抑制作用存在量效反应关系 ;在感染后 0h、2h、4h、6h、8h ,甘草均有抑制呼吸道合胞病毒的作用。结论 :甘草在Hela细胞中对呼吸道合胞病毒有明显的抑制作用 ,其作用机制是多途径的更多还原

【Abstract】 Objective:To develop safe and effective anti-RSV new medicine from Radix Glycyrrhizae.Method:The anti-RSV effect of Radix Glycyrrhizae in Hela cell culture was observed by means of the inhibition of cytopathic effect.Result:In Hela cell culture,Radix Glycyrrhizae was found to be a inhibitor of RSV in a concentration-dependent manner. The median toxic concentration(TC 50 ) of Radix Glycyrrhizae was 3.43 g/L,the median effective concentration (EC 50 ) of Radix glycyrrhizae against replication of the Long strain of RSV in Hela cells were 0.2535 g/L,the selectivity index (TI=TC 50 /EC 50 ) is 13.53.In time of addition experiment,Radix Glycyrrhizae inhibited the effect of RSV in Hela cells when it was added at 0h,2h,4h,6h,8h after virus infection.Conclusion:In Hela cell culture,Radix Glycyrrhizae was found to be a inhibitor of RSV,there are many ways in the mechanisms.更多还原