【摘要】 目的:从体外实验观察黄芪总苷(AST)对关节损伤的作用。方法:检测兔关节软骨35S掺入量和滑膜成纤维细胞的[3H]TdR掺入量。结果:AST(0.8-3.2mg·L-1)可明显上调rhIL-1(30U·mL-1)或Fe2+-H2O2体系(Fe2+和H2O2各10μmol·L-1)引起过低的兔关节软骨蛋白聚糖合成量,下调Fe2+-H2O2体系(各20 μmol·L-1)引起的过高的滑膜成纤维细胞增殖;进一步研究显示AST对OH’有直接清除作用。结论:AST对Fe2+-H2O2体系和rhIL-1 引起的兔关节损伤有保护作用,机制可能与直接清除OH’有关。更多还原

【Abstract】 Objective:To study the effect of astragalosides(AST)on joint damage in vitro. Methods: The levels of 35S incorporation in articular cartilage and the levels of 3H-TdR incorporation in synovial fibroblast from rabbits were detected. Results: In vitro treatment of AST(0.8-3.2mg·L-1) could enhance the decreased proteoglycan synthesis of articular cartilage induced by rhIL-1(30 U·mL-1)or Fe2+ -H2O2(each 10μmol·L-1)system as well as suppress increased proliferative responses of synovial fibroblasts induced by low Fe2+-H2O2 system(each 20μmol·L-1). Further study showed that AST could scavenge OH’ directly. Conclusion: AST could protect against joint damage induced by Fe2+ -H2O2 system and rhIL-1 ,which might be related to the effect of AST on scavenging OH’.更多还原