【摘要】 目的 :研究肝胃不和型功能性消化不良 (FD)大鼠幽门括约肌乙酰胆碱酯酶 (AChE)、一氧化氮合酶(NOS)表达的变化 ,探讨情志舒方对其影响及作用机制。方法 :采用夹尾刺激法制作FD大鼠模型。采用还原型辅酶Ⅱ和亚铁氰化铜直接显色的组织化学方法分别检测AChE、NOS阳性神经元成分的变化。结果 :模型组幽门括约肌AChE、NOS的表达比正常组显著减少 (P <0 .0 1) ,情志舒组与多潘立酮组比模型组显著升高 (P <0 .0 5 ,<0 .0 1) ;情志舒组疗效优于多潘立酮组 (P <0 .0 5 )。结论 :FD幽门括约肌内ACh减少 ,NO过度释放。情志舒可使幽门括约肌AChE、NOS阳性神经原成分表达升高 ,增加ACh的表达 ,抑制NO过度释放 ,增加胃肠动力更多还原

【Abstract】 Objective:To explore the expression of AchE and NOS in pyloric sphincter in rats with functional dyspepsia(FD) and to assess the effect of Qingzhishu on FD. Methods:FD rats were induced by stimulating rats on tails with forceps clip for 7 days. NADPH diaphorases and AchE histochemistry assays were used to study the distribution of NOS and AchE in the pyloric sphincter from FD rats.Results:In pyloric sphincter, the expression of NOS and AchE in model group was lower than that in normal group (P<0.01).Qingzhishu and domperidone increased the expression of NOS and AChE(P<0.05, <0.01).Compared with domperidone, the effect of Qingzhishu was more significant(P<0.05).Conclusion:Qingzhishu can significantly improve the expression of AchE and NOS in pyloric sphincter, restrain the overrelease of NO and improve the gastrointestinal motility.更多还原