【摘要】 目的探讨过氧化氢诱导鼠白内障形成过程中晶体上皮凋亡细胞中半胱氨酸天冬氨酸酶-2(Cas-pase-2)的表达及意义。方法大鼠晶状体器官离体培养后,用免疫组织化学方法检测晶体上皮细胞Caspase-2的表达。结果随过氧化氢损伤时间的处长,晶体上皮细胞Caspase-2的表达逐渐增强,与晶体混浊程度正相关。结论过氧化氢可以诱导鼠晶体上皮细胞Caspase-2的表达,后者可能参与晶体上皮细胞凋亡和白内障的形成。更多还原

【Abstract】 Objective: To investigate the expression and significance of caspase-2 of rat lens epithelial apoptosis cells during H2O2-induced cataract formation. Methods: After Vitro rat’s lens organ culture, we examined the expression of caspase-2 of rat lens epithelial cells in using immunohistochemmistry. Results: The longer incubate with H2O2, the more expression of caspase-2 of lens epithelial cells were found and the latter was positively correlating with opaque of lens. Conclusions: The expression of caspase-2 of rat lens epithelial cells can be induced by H2O2, and caspase-2 might play an important role in lens epithelial cells apoptosis and the formation of cataract.更多还原