【摘要】 目的检测73例不孕妇女宫颈分泌物中沙眼衣原体感染情况。方法应用荧光定量PCR及金标法并将两种方法作比较。结果表明用荧光定量PCR法检测不孕的沙眼衣原体阳性为41例(56.1%),阴性32例(43.9%),金标法检测阳性为19例(26.0%),阴性54例(74.0%),荧光定量PCR检测的阳性率明显高于金标法检测(P <0.01)。结论荧光定量PCR法在检测沙眼体较金标法更敏感、快速,是早期诊断生殖道沙眼衣原体感染的一种极有价值的方法。更多还原

【Abstract】 Objective: To explore the difference of examining Chlamydoza trachomatis in endocervices of women by the fluoroqenic quantitative PCR and gold-labelled antigen method. Method: Chlamydozoa trachomatis in the endocervices of women were detected by the fluoroqenic quantitative PCR and gold-labelled antigen detection method. Result: The positive rate of the 73 women by fluoroqenic quantitative PCR (56.1) was higher than that by gold-labelled antigen detection method (26%, P <0.01). Conclusion: It is suggested that PCR in detecting chlamydozoa trachomatis appears to be more sensitive and more rapid than gold-labelled antigen detection method.更多还原