【摘要】 目的　研究生物衍生骨与骨髓基质干细胞 ( marrow stromal stem cells,MSCs)复合修复山羊胫骨缺损的血管化过程 ,了解其修复长段管状负重骨缺损的血管化情况。　方法　制备生物衍生骨作为支架材料 ,培养、诱导MSCs作为种子细胞 ,二者在体外复合构建组织工程骨。 2 0只山羊双侧胫骨中段制备成 2 0 mm长的骨 -骨膜缺损模型 ,采取自身左右侧对照 ,实验侧 (右侧 )缺损处植入组织工程骨 ,对照侧 (左侧 )植入单纯支架材料 ,采用钢板内固定。术后2、4、6及 8周用墨汁灌注透明标本及血管面积图像分析方法观察血管化过程 ,组织学观察血管形成及成骨情况。　结果术后 2、4周 ,实验侧血管形成较对照侧少 ( P<0 .0 5 ) ;术后 8周 ,两侧均完全血管化 ,差异无统计学意义 ( P>0 .0 5 )。实验侧于术后 6、8周新骨形成逐渐增加 ,材料降解吸收较对照组快 ;对照侧术后 8周材料孔隙内仍无明显新骨形成。　结论　生物衍生骨作为骨组织工程的支架材料 ,能够较快发生血管化 ;组织工程骨成骨能力较单纯支架材料强更多还原

【Abstract】 Objective To study the vascularization of the composite of bio derived bone and marrow stromal stem cells(MSCs) in repairing goat tibial shaft defect. Methods Bio derived bone was processed as scaffold material. MSCs were harvested and cultured in vitro. The multiplied and induced cells were seeded onto the scaffold to construct tissue engineered bone. A 20 mm segmental bone defect in length was made in the middle of the tibia shaft in 20 mature goats and fixed with plate. The right tibia defect was repaired by tissue engineered bone (experimental side), and the left one was repaired by scaffold material (control side).The vascularization and osteogenesis of the implants were evaluated by transparent thick slide, image analysis of the vessels, and histology with Chinese ink perfusion 2, 4, 6, and 8 weeks after operation. Results More new vessels were found in control side than in experimental side 2 and 4 weeks after implantation ( P <0.05). After 8 weeks, there was no significant difference in number of vessels between two sides( P >0.05), and the implants were vascularized completely. New bone tissue was formed gradually as the time and the scaffold material degraded quickly after 6 and 8 weeks in the experimental side. However, no new bone tissue was formed and the scaffold degraded slowly in control side 8 weeks after operation. Conclusion Bio derived bone has good quality of vascularization. The ability of tissue engineered bone to repair bone defect is better than that of bio derived bone alone.更多还原