【摘要】 干旱是影响玉米生产的重要限制因素 ,特别是花期对干旱胁迫非常敏感。本研究通过对玉米L0 5 0×B73的 180个F2 :3 家系进行开花期干旱胁迫处理和分子标记鉴定 ,重点对开花相关性状进行了数量性状位点 (QTL)分析。结果表明 ,在干旱胁迫处理条件下 ,存在与出苗到抽雄天数有关的 6个QTL ,位于第 1、6、9染色体上各 1个 ,位于第 3染色体上有 3个 ,共可解释的表现型变异为 5 5 .0 % ;基于出苗到散粉天数检测到 4个QTL ,其中两个位于第 3染色体上 ,位于第 1、2染色体上各 1个 ,共可解释的表型变异为 5 2 .8% ;对出苗到吐丝天数检测到分别位于第 3、6染色体上的 2个QTL ,共可解释的表现型变异为 2 0 .4 % ;对抽雄至吐丝间隔天数 (ASI)只检测位于第 6染色体上的 1个QTL ,可解释 6 .5 %的表现型变异。而正常灌溉环境下 ,检测到出苗到抽雄天数检测到 1个QTL ,位于第 9染色体上 ,可解释的变异为 15 .0 % ;对出苗到散粉天数检测到 3个QTL ,位于第 1、3、9染色体上 ,共可解释的变异为 5 5 .0 % ;对出苗到吐丝天数检测到 4个QTL ,分别位于第 1、2、3、7染色体上。共可解释表现型变异的 4 6 .8% ;对ASI检测到分别位于第 2、6染色体上的 2个QTL ,可解释的变异为 15 .5 %。这些QTL的基因效应以显性与超显性为主更多还原

【Abstract】 A SSR genetic linkage map of maize was constructed based on the mapping population “B73 × L050” in the present study. Totally 180 F 2:3 families were grown under well watered condition and drought stress at flowering stage. Quantitative trait loci (QTL) analyses for flowering related traits were conducted for two environments. For the drought stress environment, six QTL locating on chromosome 1, 3, 6 and 9 were detected for days to tasseling, explaining 55.0% of the phenotypic variance; four QTL locating on chromosome 1, 2 and 3 for days to anthesis, explaining 52.8% of the phenotypic variance; two QTL locating on chromosome 3 and 6 were detected for days to silking, explaining 20.4% of the phenotypic variance; only one QTL was detected for anthesis to silking interval (ASI) which was localized on chromosome 6, explaining 21.3% of the phenotypic variance. For the well watered condition, one QTL was detected for tasseling which was localized on chromosome 9, explaining 15.0% of the phenotypic variance; three QTL were detected for anthesis which were localized on chromosome 1, 3 and 9, explaining 55.0% of the phenotypic variance; four QTL were detected for silking which were localized on chromosome 1, 2, 3 and 7, explaining 46.8% of the phenotypic variance; 2 QTL were detected for ASI which were localized on chromosome 2 and 6, explaining 15.5% of the phenotypic variance. Predominantly, these QTL showed overdominance or dominance, but some putative QTL had additive action.更多还原