【摘要】 目的应用我国自行研制的光学蛋白质芯片检测乳腺癌组织中的胸苷磷酸化酶 (Thymidinephosphorylase,TP) ,为建立一种快速、简便的临床检测方法进行初步探索。方法应用TP的多克隆抗体制备光学蛋白质芯片 ,通过椭偏光学显微成像技术检测20例经病理证实的乳腺浸润性导管癌组织标本和正常组织标本中的TP水平 ,以灰度值表示组织中TP浓度高低。同时应用ELISA方法测定 ,并比较两种方法检测结果的一致性。结果20例癌组织的灰度值35.40±11.55,20例正常组织的灰度值为26.80±8.78,两组间差异具显著性意义 (P<0.05)。以44.36(正常组织平均值 +2倍标准差 )为阈值 ,乳腺癌检测的灵敏度为20.00 %,特异度为100.00 %。同ELISA检测结果相比 ,在20例乳腺癌组织标本检测中 ,两种方法的一致性具极显著意义(P<0.01)。结论我国首创的椭偏光学显微成像技术简单、直观 ,结合光学蛋白质芯片可用于胸苷磷酸化酶的临床检测更多还原

【Abstract】 Objective Optical Protein Chip(OPC)created in CAS(Chinese Academy of Science)was iniˉtially used to measure the level of thymidine phosphorylase(TP)in invasive ductal breast cancer in order to set up a fast and simple clinical method.Methods The level of TP in20cases of cancer tissue and20cases of healthy tisˉsue verified by histology detection was measured using a kind of OPC on which anti-TP was fixed,then the result was shown directly by Imaging Ellipsometry system in gray way and compared with ELISA assay at the same time.Results The gray of TP in breast tissue was35.40±11.55and26.80±8.78in healthy tissue with a significant difference between them(P<0.05).According to the cutoff gray44.36(mean+2SD),the sensitivity of TP in breast cancer was20.00%and speciality was100.00%.And there is a significant concordance between OPC deˉtection and ELISA assay(P<0.01)among the20cases of cancer tissue.Conclusions The imaging ellipsometry method combining with OPC is simple,fast and visual that might enable its good clinical feasibility in TP detection.更多还原