节点文献
6-BA和氨基酸对黄瓜子叶离体培养成花的影响
Influences of 6-BA and Amino Acids on Flower Formation from Cotyledons of Cucumber in vitro
【摘要】 在基本培养基的筛选中,1/2MS培养基中附加0.10 mg.L-1 6-BA能显著提高离体黄瓜子叶的开花率,White培养基中附加2.00 mg.L-1的KT下开花率也有明显提高,但植株矮小、瘦弱,整株变黄,甚至透明化,长势极差。浓度在一定范围的6-BA(0.01~0.50 mg.L-1)对黄瓜子叶开花率影响不大,但在高浓度(0.50 mg.L-1)下,植株生长势差,开花迟,低浓度(0.01 mg.L-1)下,生长势较好。相同浓度的L-丙氨酸和L-酪氨酸均明显促进黄瓜子叶开花,且开花早;而甘氨酸对黄瓜子叶开花则有一定的抑制,开花率低。
【Abstract】 Cotyledon explants without embryonic axes from Yuexiu No.1 cucumber were cultured on differ-ent media. The results indicated that, the flowering rate was obviously increased by 1/2 MS medium supple-mented with 0.10 mg.L-1 6-BA. Although the higher flowering rate was got by White medium supplementedwith 2.00 mg.L-1 KT but the plant was small, thin and etiolation. There was not obvious differences in flowerrate on 1/2MS medium containing 0.01, 0.05, 0.10 and 0.50 mg.L-1 6-BA. The plant grew well at a lowconcentration of 6-BA but at a high concentration grew badly. The flowering rate was improved obviouslyby 1/2 MS medium supplemented with 2.00 mg.L-1 L-alanine and 2.00 mg.L-1 L-tyrosine respectively, whileglycine presented inhibition, the lower flowering was recorded.
【Key words】 cucumber; cotyledon; in vitro flowering; basic medium; 6-BA; amino acid;
- 【文献出处】 植物生理学通讯 ,Plant Physiology Communications , 编辑部邮箱 ,2004年02期
- 【分类号】S642.2
- 【被引频次】18
- 【下载频次】173