【摘要】 以普通狗牙根[Cynodon dactylon(L.)Pers.cv.‘Suncity’]颖果为外植体,以MS为基本培养基,外加浓度在2.0-6.0mg/L的2,4-D,能高频率地诱导出高质量的胚性愈伤组织,其中以4.0 mg/L为最佳。胚性愈伤组织最佳继代及分化的培养方法为:用MS+2,4-D 4.0mg/L继代1-2次,然后转入1/2 MS+2,4-D 2.0 mg/L中继代1-2次,再在无激素的1/2 MS中光照培养10 d,最后在MS+6-BA 3.0 mg/L中诱导分化,分化成苗率达31.7％。经电镜观察发现,胚性愈伤组织结构紧密,细胞较小,内容物丰富,而非胚性愈伤组织结构疏松,细胞巨大,内含一大液泡,几无细胞器。更多还原

【Abstract】 A tissue culture system for embryo-genie callus (EC) induction and plant regeneration of commonbermudagrassusingmaturecaryopsis (embryos) as explants was developed. The results showed that embryogenic calli could be induced from caryopsis with high frequency,in MS medium with 2,4-D 2.0-6.0 mg/L (Table 1), and the best concentration of 2,4-D was 4.0 mg/L. The best method for maintaining EC and tissue differentiation was to subculture EC in MS+2, 4-D 4.0 mg/L 1-2 times, follwed by subcultur-ing in 1/2 MS+2,4-D 2.0 mg/L for 1-2 times, then to transfer EC to 1/2 MS without hormone for a 10-d-preregeneration in light, followed by transferring to MS + 6-BA 3.0 mg/L for regeneration, with regeneration frequency 31.7% (Table 2). Morphological and micro-structural differences between EC and non-embryogenic callus (NEC) were observed by electron microscope (Fig.2). Ultrastructrual characteristics of the EC cells are described in this paper.更多还原