【摘要】 〔目的〕对驻滇部队感染性腹泻患者、猪和污水进行大肠杆菌O157∶H7(EO157∶H7)的分离鉴定。〔方法〕用O157免疫磁珠富集后 ,接种S -MAC琼脂平板培养 ,用MUG试验初筛 ,VIATEK3 2 全自动微生物鉴定仪鉴定 ,应用复合PCR法同时检测其三个毒力因子。〔结果〕从腹泻患者和猪中检出EcoliO157∶H7,该菌具有三个毒办因子。〔结论〕云南战区部队存在EcoliO157∶H7感染。复合PCR法的建立 ,为腹泻研究领域的病原学监测和流行病学调查提供了新手段更多还原

【Abstract】 〔Objective〕 To isolate and identify E coli O157:H7 in sufferers of diarrhea,pigs and sewage in the army in Yunnan Province.〔Methods〕 E coli O 157 ∶H 7 enriched by immune magnetic beads was inoculated into S-MAC plate,filtrated by MMG test and identified by KITEK 32 microorganism automatic identifier.Meanwhile,3 kinds of its toxin genes were simultaneously detected by multiplex PCR. 〔Results〕 E coli O 157 ∶H 7 containing the 3 kinds of toxin genes were detected in diarrhea sufferers and pigs.〔Conclusions〕 Infection of E coli O 157 ∶H 7 exists in the army in Yunnan Province.The multiplex PCR is a new means for detection and epidemiological investigation of diarrhea pathogens.更多还原