【摘要】 目的 :比较和分析人胎大小肠上皮内淋巴细胞 (iIEL)的发生、亚型分布和数量变化。材料和方法 :用免疫组织化学方法显示 6 2例小肠和 4 8例大肠CD3、CD4 、CD8、CD2 0 阳性淋巴细胞并进行体视学计数分析。结果 :小肠iIEL比大肠早一周出现 ,iIEL第 10周可见于小肠 ,第 11周可见于大肠 ;大小肠iIEL形态相同 ,位置相似 ,其数量均随胎龄而增加 ,但在各时期小肠iIEL均显著多于大肠 ,小肠iIEL约为大肠iIEL的 4 - 9倍。无论小肠还是大肠 ,均以T细胞为主 ,且以CD8+ T细胞为主 ,CD4 + T细胞很少 ;而固有层则有较多B细胞 ,且T细胞也是以CD4 + T细胞为主 ,CD8+ T细胞很少。结论 :大小肠iIEL有基本相同的发育规律 ,但发生时间和数量上有较大差异。更多还原

【Abstract】 Objective: To compare and analyze the development, subtype distribution and quantity changes of the intestinal intraepithilial lymphocyte(iIELs) between the human fetal small intestine and large intestine. Method: The CD 3+, CD 4+ , CD 8+ and CD 20+ lymphocytes were shown with immunohistochemistry and were analyzied with stereological method at 62 small intestine and 48 large intestine. Result: iIELs of small intestine appeared in the 10 th week of gestion and was earlier a week than that of large intestine. Their shape and location were same in the small intestine and large intestine, and the quantity increased with fetal development whether in small intestine or large intestine. But the numbers of iIELs in small intestine were 4 to 9 times of in large intestine at various developing stages. The iIELs were maimly T cells, especial CD 8+ T cells. There were also a few CD 4+ T cells. While in lamina propria, there were many B cells and the CD 4+ T cells were much more than CD 8+ T cells. Conclusion: The development law of iIELs was same both human fetal small intestine and large intestine, while there were significant defferences at their quantity and emergence time.更多还原