【摘要】 目的探讨SREBP1c对mGLUT1表达的影响。方法采用分子克隆技术,将SREBP1c和mGLUT1cDNA分别克隆到表达载体pSVsport和pGL3b上。SREBP1c与mGLUT1克隆载体同时或单独转染NIH3T3细胞,然后应用荧光素酶活性测定法、RTPCR及Northernblot等方法测定SREBP1c对mGLUT1的启动子活性调节及mRNA表达的影响。结果实验结果表明,SREBP1c可激活mGLUT1启动子的活性,并且可增加内源性mGLUT1的mRNA表达水平。结论SREBP1c可增强mGLUT1的表达,可能介导胰岛素对mGLUT1的调节过程。更多还原

【Abstract】 Objective To understand the possibility of contribution of SREBP-1c for mGLUT1 expression.Methods SREBP-1c and mGLUT1 cDNA clone into pSV-sport, pGL3b expression vector. The recombinant SREBP-1c and mGLUT1 transfected into NIH 3T3 cells, and confirm the mGLUT1 promoter activity and mRNA expression by the SREBP-1c with luciferase assay, RT-PCR and Northern blot.Results SREBP-1c activates the mGLUT1 promoter activity and increase the mGLUT1 mRNA expression. Conclusion SREBP-1c activates mGLUT1 expression, and may meditate mGLUT1 expression by insulin.更多还原