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卵巢运输温度对猪卵母细胞体外受精和发育的影响
Effects of Transport Temperature of Ovaries on In vitro Fertilization and Development of Porcine Oocytes
【摘要】 研究发现 ,在猪卵巢卵母细胞的体外受精和体外发育过程中 ,卵巢的运输温度对其有着重要的影响。当运输温度接近室温 (2 7± 2 )℃时 ,体外受精平均卵裂率为 5 3.0 % ,而运输温度接近体温 (36± 2 )℃时 ,平均卵裂率为 39.1% ,2组之间具有显著差异 (P <0 .0 1)。但 2组的囊胚形成率无显著差异 (P >0 .0 5 ) ,分别为 7.3%和 6 .9%。当温度降为(2 0± 2 )℃时 ,平均卵裂率下降到 32 .4 % ,与室温运输具有显著差异 (P <0 .0 1) ,而与体温运输差异不显著 (P>0 .0 5 ) ,囊胚形成率 (4 .4 % )低于以上 2组 ,但统计学上无显著差异 (P >0 .0 5 )。
【Abstract】 This experiment found that the transport temperature of ovaries had an important effects on fertilization and development in vitro of porcine oocytes. The results showed when the transport temperature was close to room temperature (27±2)℃,the cleavage rate after fertilization was 53.0% and significantly higher than that (39.1%)of the temperature(36±2)℃close to body temperature(P<0.01),but there was no significant difference on the rates of blastocytes formation between them(P>0.05).They were 7.3% and 6.9%, respectively. When the temperature was down to (20±2)℃,the average cleavage rate decreased to 32.4%, which significantly differed from that of (27±2)℃(P<0.01),but the rate was not different from that of (36±2)℃(P>05).The rate of blastocytes formation of (20±2)℃ was 4.4% and lower than those of above two groups ,however there was no significant difference among them(P>0.05).
【Key words】 ovaries; in vitro fertilization; transport temperature; cleavage rate; blastocytes; porcine;
- 【文献出处】 中国兽医学报 ,Chinese Journal of Veterinary , 编辑部邮箱 ,2004年06期
- 【分类号】S828
- 【被引频次】15
- 【下载频次】158