【摘要】 参考 Gen Bank中番鸭呼肠孤病毒 (m uscovy duck reovirus,MDRV ) S1基因序列 ,用计算机设计并合成了 1对引物 HP11、HP12 ,以此引物用 RT- PCR对番鸭呼肠孤病毒 S1基因进行了特异性扩增。结果表明 :引物 HP11、HP12能从所有供试的 4株分离毒 MDRV- MW9710、MW980 6、MW980 9、MW9810扩增出 30 0 bp S1基因 c DNA片段 ,而不能从禽呼肠孤病毒 (ARV) S1133株和番鸭胚成纤维 (MDEF)细胞培养物中扩增出任何片段 ;该 RT- PCR的检测灵敏度为 1pg的病毒核酸 ,特异性强 ,重复性好 ,对含毒细胞培养液和尿囊液只需用氯仿进行简单处理 ,即能检测出 MDRV核酸。因此认为 ,该 RT- PCR可以用于番鸭呼肠孤病毒的快速检测更多还原

【Abstract】 A pair of primers HP11,HP12 were designed according to the nucleatide sequence of S1 gene of muscovy duck reovirus(MDRV) in GenBank to amplify the S1 gene of MDRV from 4 MDRV strains MDRV-MW9710,MW9806,MW9809 and MW9810 and avian reovirus(ARV) S1133 strain and muscovy duck embryo fibroblast(MDEF) cell cultures.Only one MDRV-specific 300 bp cDNA products were amplified from all the 4 muscovy duck reovirus,but not from ARV S1133 strain and MDEF cell cultures.The detectable viral dsRNA amount of this RT-PCR was 0.01 pg.The results of 3 individual times were consistant.These results indicate that this RT-PCR can be used for rapid detection of the muscovy duck reovirus disease.更多还原