【摘要】 采用药物浓度逐渐递增的方法 ,以 0 .0 5× 10 - 6 地克珠利和 2 .0× 10 - 6 马杜拉霉素为起始诱导浓度 ,分别经过 18次和 2 0次传代 ,在实验室诱导了柔嫩艾美耳球虫 (Eimeria tenella)对地克珠利和马杜拉霉素的抗药性虫株。经抗药性检测 ,以最适抗球虫指数 (POAA)、病变记分减少率 (RL S)、相对卵囊产量 (ROP)和抗球虫指数 (ACI) 4项指标综合判定及综合抗药性指数 (GI) 2种方法共同判定 ,获得的地克珠利抗药株对 1.2× 10 - 6地克珠利具有完全抵抗力 ,而对马杜拉霉素完全敏感 ;马杜拉霉素抗药株对 7.0× 10 - 6 的马杜拉霉素具有完全抵抗力 ,而对地克珠利完全敏感。更多还原

【Abstract】 The resistance of E.tenella to the anticoccidial drugs diclazuril and maduramycin was induced experimentally.The initial concentrations of the two drugs were 0.05×10^-6 diclazuril and 2.0×10^-6 maduramycin respectively.It took 18 passages for the drug-resistant strain of E.tenella to 1.2×10^-6 diclazuril.But the drug-resistant strain of E.tenella to 7.0×10^-6 maduramycin appeared after 20 passages.The effects of drugs were assessed by POAA（percent of optimum anticoccidial activity）,RLS（reduction of lesion scores）,ROP（relative oocyst production） and ACI（anticoccidial index）.It was also evaluated by GI（global resistance index）.The results suggested that diclazuril and maduramycin did not exhibit the cross-resistance.更多还原