【摘要】 对茶薪菇原生质体的分离及再生进行研究 ,结果表明以溶壁酶的去壁效果最好 ,不同酶组合可提高去壁效果 ,酶解液在 2 0 %时原生质体释放量很高。酶解适宜温度为 30℃ ,培养 4～ 5d的菌丝酶解 3h释放的原生质体最多。以甘露醇作为渗透压稳定剂 ,原生质体的释放量及再生率均较高 ,再生率达到 5 %左右 ,经紫外线照射 2 0s,致死率即达 70 %以上更多还原

【Abstract】 A study on protoplast isolation and regeneration from mycelia of Agrocybe chaxinggu showed that a superior system of protoplast preparation was determined by using 2.0% driselase as lytic enzymes at 25℃ for 3 hours,0.6 mol/L annitol as osmotic stabilizer could give higher protoplast yield and regeneration ratio,the protoplast regenerating ratio was about 3%～5%.With 20 seconds of UV-irradiation,the death rate of Agrocybe chaxinggu protoplast was up to 70%.更多还原