【摘要】 采用阳离子交换树脂进行了回收Bt发酵废弃上清液中增效物质的初步研究 ,确定了最适的离子交换树脂。其最适回收条件为pH5,流速 1 .5倍v/h ,废弃上清液中增效物质的回收效率可达 83%。回收的浓缩上清液与去上清菌浆比例为 1 6∶1时对棉铃虫毒杀作用最强 ,表现出最好的增效作用。更多还原

【Abstract】 Different types of ion-exchange resin were used to recover potentiator from waste supernatant in the production process of Bacillus thuringiensis subsp. kurstaki strain MP-342. The static adsorption showed that D001 had the best adsorption ability and also the best releasing ability during elution. The optimized condition for recovering potentiator through ion-exchange adsorption was 1) Adsorption at pH 5, and 2) Eluting rate at 1.5 volume of resin/h. The recovery rate of the potentiator in the waste supernatant was 83% based on the inhibition rate against Escherichia coli DH5α. The concentrated supernatant showed highest potentiation rate when incorporated with no-supernatant Bt sludge at a ratio of 16∶1.更多还原