【摘要】 目的　 研究侧脑室注射肾上腺髓质素 (1nmol/kg ,3nmol/kg) ,对大鼠脑内心血管相关核团中c fos表达的影响。方法　应用Fos蛋白免疫组化技术。结果　 (1)侧脑室注射肾上腺髓质素诱发脑干的孤束核、最后区、蓝斑核、臂旁核和外侧巨细胞旁核 ,下丘脑的室旁核、视上核和腹内侧核以及前脑的中央杏仁核和外侧缰核等多个部位的心血管中枢出现大量Fos样免疫反应神经元。 (2 )降钙素基因相关肽受体拮抗剂CGRP8 37(30nmol/kg)可明显减弱肾上腺髓质素 (3nmol/kg)的效应。 结论 　肾上腺髓质素可兴奋脑内多个心血管相关核团的神经元 ,降钙素基因相关肽受体可能介导这一效应。更多还原

【Abstract】 Objective To examine the effects of intracerebroventricular (icv) administration of adrenomedullin (AM) on the expression of c-fos gene in rat brain nuclei involved in cardiovascular regulation. Methods Immunohistochemistry for Fos was applied. Results (1) Following icv administration of AM (1 nmol/kg, 3 nmol/kg), Fos-like immunoreactivity (Fos-LI) neurons were markedly increased in various brain areas of the rat, including the nucleus of the solitary tract, the area postrema, the locus coeruleus, the parabrachial nucleus and the nucleus paragigantocelluaris laterialis in the brainstem, the paraventricular nucleus, the supraoptic nucleus and the ventromedial hypothalamic nucleus in the hypothalamus, as well as the central amygdaloid nucleus and the lateral habenular nucleus in the forebrain. (2) Pretreatment with calcitonin gene-related peptide receptor antagonist CGRP_ 8-37 (30 nmol/kg) significantly reduced the effects of AM (3 nmol/kg) on the induction of Fos-LI in the brain. Conclusion Centrally administered AM may activate the neurons in the forebrain, the hypothalamus and the brainstem in part via CGRP receptors.更多还原