【摘要】 目的　探讨端粒酶活性与儿童急性髓性白血病 (AML)发病与发展的关系。方法　采用改良端粒重复序列PCR扩增 (PCR TRAP)方法检测 2 0 0 0年 11月至 2 0 0 3年 12月期间在深圳市人民医院和中南大学湘雅医院住院的 2 9例AML患儿骨髓单个核细胞化疗前后端粒酶活性的变化 ,并与 9例正常骨髓单个核细胞端粒酶活性进行比较。结果　初诊时 ,AML患儿骨髓细胞端粒酶活性 (35 5 5± 37 4 1)TPG ,较对照组 (2 36± 2 2 7)TPG明显增高 ,为对照组的 15倍 (P <0 0 1) ;化疗后 ,13例对治疗反应较好的患儿其端粒酶活性下降至 (2 6 6± 3 99)TPG ,其中10例≤ 1 5TPG。结论　AML患儿骨髓端粒酶活性明显上调 ,诱导缓解后显著降低。提示端粒酶活化与AML发展有关 ,其活性的变化对判断疾病的治疗反应和预后有重要意义更多还原

【Abstract】 Objective To study telomerase activity in mononuclear cells (MNCs) of bone marrow (BM) specimens from pediatric patients with acute myeloid leukemia(AML) at diagnosis and after chemotherapy.For comparative analysis,MNCs from control donors were analyzed.Methods Telomerase in 29 cases of children with AML were evaluated using a modified telomeric repeat amplification protocol (TRAP) assay.Results At diagnosis,high telomerase activity was detected in MNCs from AML children (35 55±37 41TPG).This exceeded by 15 fold the activity in MNCs from control donors(2 36±2 27TPG,n=9) (P<0 01).After chemotherapy,telomerase activity in MNCs from the AML children decreased to (2 66±3 99) TPG,and 10 cases of the AML children less than 1 5TPG.Conclusion Telomerase is highly upregulated in BM but decreased after induction therapy;and it is correlated with remission in AML children.Our findings suggest that telomerase upregulating plays an important role in the development of AML and may be a useful predictor for prognosis in childhood leukemia.更多还原