【摘要】 目的研究武汉市成人腹泻病人轮状病毒的感染状况及A组轮状病毒基因型的分布。方法收集2001—2002年度武汉市成人腹泻大便标本728份,用聚丙烯酰胺凝胶电泳进行轮状病毒检测,逆转录—聚合酶链反应进行A组轮状病毒基因型研究。结果和结论2001—2002年度武汉市成人腹泻病人A、B组轮状病毒检出率分别为7.83%和0.27%。A组轮状病毒以G3P[8]型为主。成人A组轮状病毒流行的季节和基因型别与婴幼儿的一致。更多还原

【Abstract】 To study the rotavirus infection in adult patients with diarrhea and the distributions of rotavirus VP7(G) and VP4(P) genotypes, a total of 728 fecal specimens were collected from adult patients with diarrhea in different districts of Wuhan, Hubei province,from January 2001 to December 2002.The rotaviruses RNA were detected by polyarcylamide gel electrophoresis, and the G and P genotypes of group A rotavirus were determined by RT PCR. The results showed that the detection rates of group A and B rotaviruses in the fecal specimens of adult patients with diarrhea were 7.83% and 0.27% respectively. The incidence of group A rotavikus in autumn and winter was higher than that in summer.In the positive specimens for group A rotavirus,the percentage of long pattern electro phenotype was 98.25%; of which two were G2+G3 genotype; while that of the short pattern electro phenotype was only 1.75%,of which was identified as G3 genotype.It was also found that G3 was the predominant genotype(87.72%), followed by G1 (3.51%) and the mixed type (7.02%). Among the P typed strains P[8]was the predominant one (92.98%), followed by P[4] (7.02%). G P combinations were also observed, in which G3P〔8〕 was the most commonly observed type (82.46%), followed by G3P〔4〕(5.26%). The unusual G P combination G3P 〔4〕, (G2+G3)P〔8〕and (G2+G3)P〔4〕, were also identified as well. It concludes that the predominant G P combination in Wuhan from 2001 to 2002 is G3P〔8〕,and the epidemic season and genotype of group A rotavirus in adults are all just the same as those in infant and children.更多还原