【摘要】 目的 :克隆Survivin编码序列并在真核细胞中表达。方法 :RT -PCR扩增Survivin编码序列 ,建立与绿色荧光蛋白(GFP)共表达的融合重组质粒 ,转染哺乳动物细胞 ,利用GFP绿色荧光和WesternBlot检测Survivin蛋白的表达。结果 :RT -PCR扩增出 4 2 1bp的Survivin编码序列 ,构建融合重组质粒pEGFP -C1-Survivin ,利用GFP荧光和WesternBlot证实Sur vivin蛋白的表达。结论 :成功克隆Survivin基因并在真核细胞中表达。更多还原

【Abstract】 Objective:To clone and express survivin gene in mammalian cells.Methods:Coding sequence of survivin gene was amplified from HepG 2 cell mRNA by RT-PCR and then cloned into eukaryotic vector pEGFP-C1.The vector with survivin gene was transfected into HepG 2 and SMMC-7721.The expression of survivin was detected by GFP and Western blot.Results:The recombinant plasmid pEGFP-C1-survivin was successfully constructed and expressed in HepG 2 and SMMC-7721.Conclusion:The result shows expression of the anti-apoptosis gene survivin in mammalian cells.更多还原