【摘要】 应用气相色谱-质谱(GC/MS)选择离子监测(SIM)方式探讨甾体类药物7-甲基异炔诺酮(Tibolone)的检测方法。根据Tibolone的代谢规律,对受试者的阳性尿和空白尿检测结果进行比较。研究发现:在阳性尿中检出△4双键异构体、3α羟基和3β羟基等三种代谢物,6位、16位的羟基化代谢物没有检出。测定了3α羟基代谢物的浓度-时间关系曲线,表明Tibolone口服后吸收迅速,9小时后即可达到峰值浓度。可为该类药物的检测提供科学依据。更多还原

【Abstract】 Gas chromatography-mass spectrometry (GC/MS) with selective ion monitoring (SIM) mode was applied to study the detection method of metabolites of Tibolone in human urine. According to the rule of metabolism, the experimental data obtained from masculine urine and blank urine of the tested were compared. The results indicated Δ4 double-bond i-someride,3α- hydroxyl group and 3β- hydroxyl group are detected, 6- and 16-hydroxylated metabolites not detected. The excretion curve of time-concentration of 3p-hydroxylated metabolite was investigated, the study showed that Tibolone was absorbed quickly and its concentration reached to peak value after oral for 9 hours. These features can be applied to the establishment of scientific basis for the detection of this kind of doping.更多还原