【摘要】 目的　探讨以腺病毒介导的肌红蛋白的表达对肝脏的ATP水平的影响及其在肝脏缺血再灌注 (I R)的保护作用。方法　将携带有人体肌红蛋白基因的腺病毒转染至白鼠的肝脏 ,然后检测肌红蛋白的表达 ,同时检测肝脏的ATP水平和肝功能。对肌红蛋白对肝脏I R损伤动物模型的保护效应进行评价。结果　腺病毒介导的肌红蛋白可转染至白鼠肝脏。转染 72h后白鼠肝脏的ATP水平较对照组明显升高。肌红蛋白的表达能减轻肝脏的I R损伤。结论　肌红蛋白在体肝脏的表达可提高肝脏的ATP水平 ,并可能成为减轻I R损伤的新的治疗方法。更多还原

【Abstract】 Objective To study the effect of expression of myoglobin which mediated by adenovirus,on ATP value of liver and the protective effect on liver ischemia reperfusion injury.Methods Adenovirus carrying CMV promoter sequences linked to the human myoglobin gene(AdCMVMyo) were transfected into rats liver. Then myoglobin, hepatic ATP levels and liver function were evaluated. Results Myoglobin expression was verified in rat livers after AdCMVMyo transfection. The ATP levels in rat livers 72 hours after AdCMVMyo transfection were significantly higher than that in control group(P<0.05). Moreover, expression of myoglobin attenuated ischemia-reperfusion injury of the rat liver. Conclusions Myoglobin gene transfer to the liver can augment hepatic ATP levels and might be a novel modality to reduce ischemia-reperfusion injury.更多还原