【摘要】 以桃品种‘京玉’、‘美味’的正反交F1代群体69株为试材,采用RAPD、AFLP技术与BSA相结合的方法筛选与桃果实非酸/酸性状连锁的分子标记。在筛选的301个RAPD引物中,只有引物S332在两基因池间产生的多态性片段与非酸性状连锁,但重组率高达28.9%。通过128对AFLP引物组合在两基因池间的分析结果表明,18对引物组合产生多态性。单株验证结果表明,只有3对引物组合E-ACT/M-CAT、E-TA/M-CTC和E-AT/M-CTA产生的多态性片段与果实非酸/酸性状连锁,连锁距离分别为16.2、1.47和2.99 cM。利用获得的连锁标记,构建D/d基因连锁群,连锁距离最近的2个标记AFTA-CTC、AFAT-CTA分别位于D/d基因位点两侧。更多还原

【Abstract】 In this research molecular markers linked to non-acid /acid trait in peach fruit were detected with RAPD and AFLP technique coupled with BSA method in 69 F1 population hybrid from the intra-specific cross between peach cultivars Jingyu?and Flavortop? Of 301 random primers, only the pirmer S332 produced a polymorphic band linked to non-acid fruit trait, high recombination ratio of specific fragment was 28.9%. Among 128 pairs of AFLP primer combination, 18 pairs produced polymorphism between two bulks. The result of individual amplification showed that only three of them amplified specific fragments linked to fruit non-acid/acid trait. The specific fragments derived from primers combination E-ACT/M-CAT, E-TA/M-CTC, and E-AT/M-CTA, the linkage distance were 16.2, 1.47, and 2.99 cM, respectively. The D/d gene linkage groups was established, and two nearest markers AFTA-CTC and AFAT-CTA located in two sides of D/d locus, respectively.更多还原