【摘要】 中双 9号是采用复合杂交和小孢子培养技术选育的双低甘蓝型油菜新品种 ,具有高抗菌核病、高抗倒伏、丰产性突出、稳产性好、品质优良、适应性广等优点。 2 0 0 2年油菜中双 9号通过湖北省农作物品种审定委员会审定。在 2 0 0 0～ 2 0 0 2年湖北省区域试验中该品种平均单产 2 4 82 .2kg·ha-1,比对照中油 82 1增产 15 .33%。中双 9号种子芥酸含量 0 .2 3% ,商品籽硫甙含量 2 2 .6 9μmol·g-1(饼 ) ,含油量 4 2 %。经大田自然侵染鉴定 ,中双 9号平均菌核病发病率为 13.31% ,比对照中油 82 1降低 2 8% ,病情指数为 6 .4 7,降低 36 %。经离体叶菌丝接种鉴定 ,中双 9号病斑面积仅为 4 .70 9cm2 ,显著小于中抗品种中油 82 1(5 .933cm2 ) ;经牙签接种鉴定 ,中双 9号病斑扩展长度仅为1.2 75cm ,极显著小于中抗品种中油 82 1(1.94 3cm)。还研究了不同抗性油菜品种在接种菌核病菌丝后植株体内苯丙氨酸解氨酶、几丁质酶、β 1,3 葡聚糖酶、过氧化物酶、多酚氧化酶活性变化规律 ,探讨了中双 9号抗菌核病的可能机制更多还原

【Abstract】 Zhongshuang 9, a new semi-winter Brassica napus variety with high resistance to Sclerotinia sclerotiorum and lodging, high-yield, "double-low" quality and extensive adaptability, was bred by multiple crossing and microspore culture technique. It was registered and released in China in 2002.In Regional Trial of Hubei Province in China, Zhongshuang 9 yielded 2 482.2 kg·ha -1 averagely in 2000-2002, 15.33% higher than the control variety Zhongyou 821. Erucic acid, glucosinolates and oil contents of Zhongshuang 9 were 0.23%, 22.69 μmol·g -1 (in meal) and 42%, respectively. In field identification of resistance to S.sclerotiorum, disease incidence and disease index of Zhongshuang 9 were averagely 13.31% and 6.47, respectively, which decreased by 28% and 36%, respectively,as compared with those of the control variety Zhongyou 821. By inoculation of detached leaves with mycelia, the lesion size of Zhongshuang 9 was 4.709 cm 2, which was significantly smaller than that of the mid-resistant variety Zhongyou 821( 5.933 cm 2). The lesion length of the stem of Zhongshuang 9 was 1.275 cm, which reached significant difference compared with that of the mid-resistant variety Zhongyou 821 (1.943 cm) by the Match-stick Inoculation Method. The possible mechanism of resistance to S. sclerotiorum was studied through comparing the activities of phenylalanine ammonia lyase (PAL), exo-chitinase, β-1,3-glucanase, peroxidase (POD) and polyphenoloxidase (PPO) of Zhongshuang 9 with those of other resistant, mid-resistant and susceptible cultivars.更多还原