【摘要】 mRNA差异显示技术是分子生物学中分离g隆基因的有效技术。笔者叙述了mRNA差异显示技术的基本原理、存在的问题以及技术改进, 并主要阐述了该技术在分离水稻基因中的应用情况。更多还原

【Abstract】 mRNA differential display reverse transcription polymerase chain reaction(DDRT-PCR)is an effective technology in isolation and cloning genes in molecular biology. The primary principle, superiorities, shortages, and technological improvements were reviewed, and this paper mainly focuses on the application of the technology in the isolation of rice (Oryza sativa L.) genes.更多还原