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雅致放射毛霉胞外蛋白酶特性的研究
Research on characteristics of extra-cellar protease of Actinomucor elegans 3.27
【摘要】 对雅致放射毛霉分泌的胞外蛋白酶进行了特性研究。发现该胞外蛋白酶是以酸性蛋白酶为主、含有中性和碱性蛋白酶的复合酶系,其中中性蛋白酶对酸碱和温度的稳定性好,在腐乳后酵中起主要作用,最适作用温度为55℃,适量的NaCl(<2%)、CaCl2(0.08%)和MgCl2(0.08%)对该蛋白酶具有激活作用。并结合腐乳生产,对缩短后酵提出了建议。
【Abstract】 This paper investigated the characteristics of extra-cellular protease of Actinomucor elegans 3.27. The results showed that the system included acid, neutral and alkaline protease, among which the neutral protease was the most stable to temperature and acidity and the dominant protease in sufu ageing. The optimal temperature of protease activity was 55℃ and the correct concentration of NaCl (<2%), CaCl2 (0.08%) and MgCl2 (0.08%) could make it active.
【基金】 安徽省青年教师基金资助([02]2003jP161)
- 【文献出处】 中国酿造 ,China Brewing , 编辑部邮箱 ,2004年12期
- 【分类号】TS214.2
- 【被引频次】23
- 【下载频次】252