【摘要】 目的 :用榄香烯复合瘤苗抗原 卡介苗热休克蛋白复合物 (HTA HSP70 BCG)冲击正常小鼠骨髓树突状细胞 ,检测其抗原递呈功能。方法 :用HTA HSP70 BCG刺激 ,并与GM CSM和IL 4联合培养的骨髓树突状细胞 ,用MTT法测增殖活性 ,用透射电镜检查形态学改变 ,用流式细胞仪检测FITC labeleddextran的摄取。结果 :与单用HTA或HSP70 BCG刺激的树突状细胞相比 ,HTA HSP70 BCG刺激的树突状细胞增殖指数明显增大 (2 10 7± 0 0 13) ,对脾不粘附细胞的激活作用明显增强 (增殖指数为 1 92 7±0 0 73) ,FITC labeleddextran摄取率明显增高 (达 5 8 6 1% ) ,形态上更趋于成熟。结论 :HTA HSP70 BCG 对树突状细胞具有较强的冲激作用 ,增强其抗原递呈功能。更多还原

【Abstract】 Objective:Normal murine DCs were pulsed with complex of tumor antigen from elemene-combo tumor cell vaccine-heat shock protein 70 of BCG (H TA-HSP70 BCG).Their proliferation and antigen presenting function were evaluated.Methods:The dendritic cells(DCs)were cultured in complete media containing GM-CSF and IL-4 and pulsed with H TA-HSP70 BCG,H TA or HSP70 BCG.Their proliferation and stimulating effects on spleen nonadherent cells were evaluated with MTT assay.Their capability of endocytosing FITC labeled dextran was assayed with FACscan,morphological changes of DC were observed in electron microscope.Results:Proliferation index of DCs pulsed with H TA-HSP70 BCG was 2.107±0.013,proliferation index of DCs pulsed with H TA-HSP70 BCG mixed with normal nonadherent spleen cell was 1.927±0.073.The percent of DCs endocytosed FITC labeled dextran was 58.61%.Above changes were more significant than those of DCs pulsed with H TA or HPS79 BCG.Conclusion:H TA-HPS70 BCG had more potent activity to pulse DC and strengthen antigen presenting of DC.更多还原