【摘要】 目的 :定位NZB、NZW及NZB WF1小鼠外周血B1细胞增殖的易感基因。方法 :建立 (NZB×NZW )F1×NZB回交小鼠模型 ,用覆盖小鼠全部常染色体的多态性微卫星遗传标记数量性状位点 (QTL)分析进行基因定位。结果 :B1细胞增殖的易感基因与小鼠的第 2、4、17号染色体上的微卫星遗传标记D2Mit 10 1、D4Mit 11及D17Mit 6 1肯定连锁 (Lods值 >3) ,其中D2Mit10 1、D4Mit11位点来源于NZB ,D17Mit 6 1位点来源于NZW。在D2Mit10 1位点附近存在Ltk、Rag1、2基因 ,在D4Mit 11位点附近存在Lck基因 ,在D17Mit6 1位点附近存在H 2及TNF α基因。结论 :NZB、NZW及NZB WF1小鼠外周血B1细胞增殖受多基因调控 ,易感基因分别位于NZB的Ltk、Rag1、2及Lck基因编码区和位于NZW的H 2、TNF α基因编码区 ,这些易感基因之间有相互促进的叠加效应。更多还原

【Abstract】 Objective:To map the susceptibility genes for peripheral B1 cell proliferation in New Zealand mice.Methods:The authors set up(NZB×NZW)F1×NZB backcross mice model and used polymorphic microsatellite markers and quantitative trait locus(QTL)analysis.Results:Susceptibility genes of B1 cell proliferation was linked to the microsatellite markers on chromosome 2,4 and 17,such as D2Mit 101,D4Mit 11 locus derived from NZB and D17Mit 61 locus derived from NZW,nearby which there were Ltk,Rag 1,2,Lck,H-2 and TNF-α genes according to the QTL analysis.Conclusion:The peripheral B1 cell proliferation in New Zealand mice is controlled by multiple genes.Susceptibility genes are located at Ltk,Rag 1,2 and Lck gene coding regions of NZB and at H-2,TNF-α gene coding regions of NZW.There are additive effects between these susceptibility genes.更多还原