【摘要】 目的　探讨骨桥蛋白 (OPN)对血管平滑肌细胞是否具有促黏附及趋化作用。方法　从大鼠源性血管平滑肌细胞的细胞培养基中纯化OPN ,制备家兔抗OPN多克隆抗体。用酶联免疫吸附及迁移分析方法测定OPN对血管平滑肌细胞的影响。结果　骨桥蛋白能够促进血管平滑肌细胞发生黏附 ,且其促黏附功能呈浓度依赖性。黏附率在实验开始后 90～ 1 2 0min区段达到最高。与对照相比 ,OPN刺激血管平滑肌细胞的迁移距离明显增加。结果　骨桥蛋白是血管平滑肌细胞的黏附、迁移活化因子更多还原

【Abstract】 Objective To explore whether osteopontin (OPN) is an functional adhesive and chemotactic molecule for vascular smooth muscle cells (VSMCs).Methods OPN was purified from cultured medium of VSMCs of rats and was used to produce the polyclonal antibodies against OPN of rabbit. The effect of OPN on the VSMCs was detected with ELESA and migration analysis methods. Results OPN could promote the adhesion of VSMVs in a dose dependent manner. We found that the maximal number of adherent cells occurred at 90～120 minutes in the assay. Migration distance of VSMCs stimulated by OPN was longer compared with control group. Conclusions OPN can promote the adhesion and migration of VSMCs significantly更多还原