【摘要】 目的　研究抑癌基因PTEN在肝细胞癌 (HCC)中的表达及其与蛋白激酶B(PKB/AKT)磷酸化的相关性。方法　采用免疫组织化学S P染色法 ,Western印迹杂交法检测 35例肝细胞癌 ,15例肝硬变 ,8例正常肝组织中PTEN的表达 ,并分析肝癌组织中PTEN表达与AKT磷酸化水平的关系。结果　肝癌组织中PTEN蛋白的阳性表达 (6 2 .9% ,0 .0 85± 0 .0 2 1)明显低于肝硬变和正常肝组织 (P <0 .0 1) ;同时低分化、有肿瘤转移的肝癌其PTEN表达强度明显低于高分化、无转移的肝癌 (P <0 .0 5 ) ;相关性分析表明 :肝癌组织中PTEN蛋白表达和AKT磷酸化水平呈明显负相关 (r =- 0 .818,P <0 .0 1)。结论　由于PTEN蛋白表达下调或缺失 ,而导致AKT磷酸化水平的明显增高 ,在肝癌的发生、发展中起着重要的作用。更多还原

【Abstract】 Objective To investigate clinical significance of phosphatase and tensin homolog deleted on chromosome ten (PTEN) protein exppression and correlation between the expression of PTEN and phosphorylation of protein kinase B (PKB/AKT) in human hepatocellular carcinoma (HCC). Methods The expression of PTEN and phospho-AKT were detected by SP immunohistochemical technique and Western blotting in 35 cases of HCC, 15 cases of liver cirrhosis and 8 cases of normal tissues. Results The positive expression of PTEN in HCC ( 62.9%, 0.085± 0.021) were significantly lower than those in liver cirrhosis and normal tissues (P< 0.01). The expression level of PTEN was related to the differentiation degree of HCC and the status of metastasis (P< 0.05). Western blotting revealed a significant inverse correlation between PTEN and phospho-AKT (r= -0.818, P< 0.01). Conclusion These results demonstrate that down-regulation of PTEN, which may not be able to effectively inhibit the hyper-phosphorylation of PKB/AKT, will be an important step in tumor genesis and progression of hepatocellular carcinoma.更多还原