【摘要】 目的探讨异丙肾上腺素诱导的心肌损伤和损伤过程中细胞凋亡相关基因fas和bcl-2的表达改变及比索洛尔对其的预防作用。方法实验分为3组:A组:WS大鼠20只,腹部皮下每天注射异丙肾上腺素1mg?kg;B组:WS大鼠20只,预先予比索洛尔2mg?kg?d灌胃,3d后加用异丙肾上腺素1mg?kg?d皮下注射;C组:WS大鼠12只,腹部皮下注射生理盐水1ml?d。3组均腹部皮下注射10d。结果A组与C组相比,心肌组织坏死明显,fas和bcl-2的表达上调(均P<0.01)。B组心肌组织的HE病理损害等级明显减轻(χ2=10.36,P<0.01),fas蛋白表达明显下降,bcl-2蛋白表达明显上调(均P<0.05)。结论在异丙肾上腺素诱导下的大鼠心肌损伤过程中fas和bcl-2表达水平明显升高,心肌损伤明显;比索洛尔可以有效抑制异丙肾上腺素引起的心肌损伤,抑制凋亡促进基因fas的表达,相对上调凋亡抑制基因bcl-2的表达。更多还原

【Abstract】 Objective To observe the protective effects of bisoprolol on the d myocardium injury induced by isoproterenol (ISO) in rats and its mechanism. Methods The rats were divided into 3 groups: in group A (n=20), 1mg/kg/d ISO was injected subcutaneously; in group B (n=20), rats were fed with bisoprolol (2 mg/kg/d) for 3 day before ISO was injected (same as group A); in group C (n=12) normal saline (1ml/d) was injected subcutaneously. Result In group A, necrotic foci of myocardium was predominant microscopically, expression of fas and bcl-2 incresed comparing with those in group C (P<0.01). In group B, the necrotic foci were alleviated comparing with group A (χ2=10.36, P<0.01), expression of fas decreased, while bcl-2 increased significantly(P<0.05). Conclusion ISO can cause necrosis of myocardium, and over-expression of fas and bcl-2 in rats; bisoprolol can effectively alleviate the myocardium damage induced by ISO.更多还原