【摘要】 目的 :探讨滋养细胞疾病核苷酸还原酶小亚单位 (R2 )的表达水平及其与疾病预后间的关系。方法 :采用免疫组织化学 S- P法检测 15例正常绒毛、38例葡萄胎、4 2例侵蚀性葡萄胎和 18例绒毛膜癌组织中 R2蛋白的表达。结果 :葡萄胎、侵蚀性葡萄胎、绒毛膜癌的 R2表达水平显著高于正常绒毛 (P=0 .0 0 0 ) ;葡萄胎、侵蚀性葡萄胎和绒毛膜癌之间的表达无差异 ;8例葡萄胎恶变患者 R2水平显著高于自然恢复者 (P=0 .0 2 )。术前未化疗与术前化疗的侵蚀性葡萄胎及绒毛膜癌患者 R2蛋白表达均无差异。 WHO 期及 期的滋养细胞肿瘤患者 R2蛋白表达显著高于 期者 (分别 P=0 .0 2 3,P=0 .0 38) ,WHO预后评分为中危和高危的滋养细胞肿瘤患者 R2表达显著高于低危者 (分别 P=0 .0 18,P=0 .0 0 6 )。结论 :R2在滋养细胞疾病中的表达增加 ,可能与滋养细胞的增生、葡萄胎恶变和滋养细胞肿瘤的高危耐药等生物学行为有关更多还原

【Abstract】 Objective: To study the expression of the small subunit ribonucleotide reductase(R2) in gestational trophoblastic diseases (GTD) and to assess its prognostic value. Methods: The expression of R2 was detected with immunohistochemical method in 15 cases of normal villi, 38 cases of hydatidiform mole (HM), 42 cases of invasive moles (IM) and 18 cases of choriocarcinoma (CC). Results: R2 expression in HM, IM and CC was significantly increased compared with that of normal villi ( P =0 000). There were no significant differences in R2 protein expression among HM, IM and CC. Among 38 cases of HM, R2 expression in 8 cases with malignant transformation was significantly higher than in 30 cases of non malignant transformation mole ( P =0 02). Preoperative chemotherapy of gestational trophoblastic tumor including IM and CC did not influence the R2 expression. Compared with patients of stageⅠ( WHO),the R2 protein in gestational trophoblastic tumor (GTT) patients of stageⅢ or stageⅡ was significantly increased ( P =0 023 and P =0 038,respectively). The value of R2 in GTT patients with middle or high risk in WHO prognostic scoring system was higher than in the patients with low risk ( P =0 018 and P =0 006,respectively). Conclusion: R2 expression in GTD is increased, which may be associated with the hyperplasia of trophoblasts, malignant transformation of hydatidiform mole and drug resistance of trophoblastic tumor.更多还原