【摘要】 目的 :研究透明质酸酶联合 C2 F6玻璃体腔注药诱导兔眼玻璃体后脱离 (PVD)的效果及安全性。方法 :15只兔 (30只眼 )随机分 A、B、C 3组 ,每组 5只 ,每兔一眼为实验眼 ,另一眼为对照眼。A、B两组实验眼玻璃体腔注射 30 IU透明质酸酶 ,C组实验眼玻璃体腔注入 0 .1ml BSS溶液 ,注射后第 7d A组和 C组加注 C2 F60 .5 ml,对照眼均注射 0 .1ml BSS溶液。注射后前 10 d每天行临床裂隙灯、前置镜、检眼镜及 B超和暗适应视网膜电图 (ERG)检查 ,以后除每天常规临床检查外 ,每周复查 B超及 ERG,共观察 8周。处死后眼球标本送光镜和扫描、透射电镜检查。结果 :A组 5只实验眼完全性玻璃体后脱离 ,B组 3只实验眼不完全性玻璃体后脱离 ,C组实验眼和 3组对照眼均无玻璃体后脱离发生。 A、B、C组实验眼注药前后及与对照眼相比较 ,视网膜电图 a、b波振幅和潜伏期均无显著改变 (P>0 .0 5 )。光镜、电镜检查未发现视网膜组织有毒性改变。结论 :动物实验中临床观察结合 B超、扫描电镜有助于准确判断玻璃体后脱离与否。透明质酸酶联合 C2 F6玻璃体腔注药可诱导兔眼完全性玻璃体后脱离的发生 ,但无眼内毒性更多还原

【Abstract】 Objective: To evaluate the experimental induction of posterior vitreous detachment （PVD） by intra-vitrous injection of hyaluronidase and perfluoroethane （C₂F₆）. Methods: Fifteen rabbits （30 eyes） were divided into 3 experimental groups,the contralateral eyes in same animals served as the controls. Eyes in group A and B were received two vitreous injections of 15 IU of hyaluronidaseat an interval of 5 d. The eyes in group C and all control eyes were injected with balanced salt solution （BSS）. Seven days after injection,the experimental eyes in group A and C were received 0.5 ml of C₂F₆ injection. The ocular and retinal signs were examined for 8 following weeks and then killed for histological examination. Results: Five eyes in group A （100.0%） showed complete se-（paration） of the vitreous cortex from the retina （PVD）,three eyes in group B（60.0%） showed partial PVD,and no PVD was detected in group C and all control eyes. On electroretinogram no significant difference was found in amplitude and latency of a- （or b-） wave in both experimental and control eyes,between before and after experiments. No （evidence） of ocular or retinal toxicity was revealed by light or scanning electronic microscopy in all eyes. Conclusions: Vitreous injection of hyaluronidase combined with perfluoroethane,as a safety method,can induce posterior （vitreous） detachment without mechanical vitrectomy.更多还原