【摘要】 目的 :探讨浙江汉族人群中血管紧张素原 (AGT)基因 M2 35 T变异与原发性高血压的关系。方法 :对116例高血压病患者与 114名正常血压者进行对照研究。采用聚合酶链式反应 (PCR)与限制性片段长度多态性(RFLP)技术检测 AGT基因 M2 35 T变异。同时测量血压、身高、体重、空腹血糖与血脂。结果 :1高血压组收缩压与舒张压水平显著高于对照组 (P<0 .0 1) ;而两组间年龄、性别、体重指数、血糖与血脂差异无显著性 (P>0 .0 5 )。2经 PCR扩增及 Tth 111I酶切 ,AGT基因型有三种形式 :MM、TT与 MT基因型。两组 AGT基因型的分布均符合 Hardy- Weinberg平衡。 3AGT基因 M2 35 T基因型在高血压组与对照组的分布差异有显著性 (χ2 =6 .96 6 ,P<0 .0 5 )。高血压组 TT基因型与 T2 35等位基因的频率高于对照组 (TT基因型 :0 .4 7vs 0 .33,χ2 =5 .36 ,P<0 .0 5 ;T2 35等位基因 :0 .71vs 0 .6 0 ,χ2 =6 .179,P<0 .0 5 )。结论 :在浙江汉族人群中 ,AGT基因 M2 35 T变异与原发性高血压的发病显著相关 ,TT基因型或 T2 35等位基因可能是高血压的一个遗传方面的危险因素。更多还原

【Abstract】 Objective: To investigate the association of the AGT gene M235T variant with essential hypertension in Han population of Zhejiang Province. Methods: The study included 230 subjects: 116 hypertensive patients and 114 normotensive controls. Polymerase chain reaction(PCR)combined with restrictive fragment length polymorphism(RFLP)was used to detect the M235T variant of angiotensinogen (AGT) gene . Blood pressure, body height and weight, fasting blood glucose and serum lipid were measured in all subjects. Results: ①The systolic blood pressure and diastolic blood pressure of hypertensive group were significantly higher than those of control group, while no significant difference was observed with regard to age, gender, body mass index, blood glucose, or lipid profile. ②The genotype distribution of AGT gene in both groups was in agreement with Hardy-Weinberg equilibrium. ③The genotype distribution of the M235T variant differed significantly in hypertensives and controls(χ2=6.966,P<0.05). The frequencies of genotype TT and T235 allele in hypertensives were higher than those in controls(TT genotype: 0.47 vs 0.33, χ2=5.36,P<0.05; T235 allele: 0.71 vs 0.60, χ2=6.179, P<0.05). Conclusions: The molecular variant M235T of the AGT gene is significantly associated with essential hypertension in this population. The genotype TT or allele T235 might be a genetic risk factor for hypertension.更多还原