【摘要】 目的　从噬菌体肽库筛选旋毛虫抗原的模拟表位 ,为研究和开发新的旋毛虫病诊断抗原提供实验依据。方法　以纯化旋毛虫病患者血清免疫球蛋白 (Ts IgG)对噬菌体十二肽库进行 5轮筛选 ,随机挑选 2 4个克隆 ,通过ELISA选取吸光值 (A)较高的 6个克隆 (T1～T6) ,并检测其敏感性和特异性。　结果　T1～T6敏感性与旋毛虫幼虫抗原 (TsA)相同 (阳性率为 10 0 % ,P >0 .0 5 ) ,特异性与TsA无明显差异 (阴性率为 0～ 40 % ,P >0 .0 5 ) ,其中T3 ,T6与并殖吸虫病患者血清无交叉反应 ,特异性高于TsA(P <0 .0 5 ) ,T6与日本血吸虫病患者血清无交叉反应 ,特异性亦高于TsA(P <0 .0 5 )。　结论　利用噬菌体十二肽库筛选到旋毛虫抗原模拟表位更多还原

【Abstract】 Objective To screen special mimic epitopes of Trichinella spiralis antigen from peptide library for exploring new diagnostic antigens. Methods Ts-IgG purified from serum of trichinosis patients was used to screen the phage 12-mer peptide library for 5 rounds. 24 clones were picked out randomly to detect the immunoactivity. The sensitivity and specificity of the 6 clones (T1-T6) whose A values were higher than others were tested by ELISA. Results The sensitivity of the clones T1-T6 was the same with larval antigen of Trichinella spiralis (TsA) (positive rate: 100%, P>0.05), and there was no difference in specificity between T1-T6 and TsA (negative rate: 0-40%, P>0.05); T3 and T6 did not react with sera from patients of paragonimiasis, showing higher specificity than TsA(P<0.05); T6 did not react with sera from patients of schistosomiasis, also showing higher specificity than TsA(P<0.05). Conclusion The mimic antigenic epitopes of Trichinella spiralis have been successfully obtained by screening phage 12-mer peptide library.更多还原