【摘要】 目的　探讨干扰素 γ(IFN γ)对小鼠抗日本血吸虫感染保护力的机制。　方法　采用高密度寡核苷酸芯片 (Affymetrix芯片 )结合半定量逆转录 聚合酶链反应 (RT PCR )方法 ,对BALB/c小鼠感染日本血吸虫过程中脾脏CD4+ T细胞进行基因转录水平分析 ,获得IFN γ诱导鸟苷三磷酸 (GTP)酶家族成员的变化图谱 ;并对其中IFN γ诱导GTP酶 (IGTP)进行分子克隆和序列测定。　结果　小鼠自然感染日本血吸虫过程中 ,IFN γ诱导GTP酶家族成员的基因表达呈现特征性变化 ,感染后 3wk ,基因表达上调或变化不显著 ;感染后 6wk至 13wk ,基因表达持续受到抑制。这种变化特征经RT PCR方法所证实。从小鼠脾脏可扩增出IGTP全长基因 ,但退火温度降低时出现IGTP基因转录缺失。　结论　小鼠急性感染日本血吸虫后 ,体内IFN γ通路逐步受到抑制 ,针对血吸虫感染的依赖IFN γ的抗感染保护力下降更多还原

【Abstract】 Objective To investigate the molecular characteristic of interferon_γ mediating protective immunity against schistosomiasis japonica in mice. MethodsCD4++ T cells were isolated from spleens of mice infected with Schistosoma japonicum at different time_points. The cDNA microarray technique combined with RT_PCR was used to explore IFN_γ inducible GTPase family gene expression profile of CD4++ T cell. IGTP, a representative IFN_γ inducible GTPase having vital anti_infection activity, was amplified from spleen of BALB/c mice using RT_PCR, then cloned into pGEM(r)_T easy vector for sequencing. Results IFN_γ inducible GTPase family had the similar characteristic over the course of S.japonicum infection. The gene expression of these members were up_regulated or had little change at 3 wk post_infection, then down_modulated from 6 wk to 13 wk post_infection, which was also confirmed by RT_PCR. As for IGTP, two inserts were identified after sequencing. One was 142 bp shorter than another, but the fragment was lost due to low annealing temperature. Conclusion There is a dramatic inhibition of IFN_γ pathway and IFN_γ_dependent anti_infective immunity during the infection of S.japonicum.更多还原