【摘要】 从杜长嘉猪的骨髓中提取基因组RNA,用反转录-聚合酶链式反应(RT-PCR)扩增抗菌肽Protegrin-1(PPG-1)基因,获得一条565bp的片断,以pGEM-TEasyvector为载体,将该基因片断克隆到大肠杆菌中.从筛选到的阳性克隆中分离出Protegrin-1基因,测定其序列,并由此推导出氨基酸序列.结果表明:1该片断长565bp,为Protegrin-1基因cDNA的部分序列,序列同源性比较表明,该基因序列与GeneBank登录的Protegrin-1基因序列具有较高的同源性,达到98.8%.2根据所测序列推导其成熟肽由18个氨基酸残基组成,其氨基酸序列与GeneBank登录的Protegrin-1的成熟肽序列完全一致.更多还原

【Abstract】 Genome RNA was extracted from bone marrow of pig (Duroc╳Landrace╳Tai-hu) and Protegrin-1 (PPG-1) mRNA was amplified using RT-PCR. A DNA fragment about 565 bp in length was obtained and the PCR product was cloned into pGEM-T vector. Protegrin-1 gene was isolated and sequenced from the positive clones screened and the corresponding amino acid sequence was deduced from its nucleotide sequence. Result of sequence analysis showed that this fragment was the partial sequence of PPG-1 gene cDNA. Its homology with PPG-1 from Genebank was up to 98.7%.The deduced sequence for the mature peptide was composed of 18 amino acid residues, which was consistent with that of porcine PPG-1 in Genebank.更多还原