【摘要】 目的探讨乙酰肝素酶基因(HPAmRNA)在胃癌中的表达及其与临床病理因素之间的相互关系。方法选用43例胃癌组织和10例癌旁正常胃组织,用逆转录聚合酶链反应(RTPCR)检测标本中HPAmRNA的表达,并结合患者临床病理指标进行分析。结果43例胃癌中,29例HPAmRNA表达阳性,其阳性率显著高于癌旁正常胃组织(P=0.013)。HPAmRNA的表达与TNM分期、有无浆膜浸润、淋巴结转移、远处转移以及与肿瘤大小有相关性(P<0.05);与患者年龄、性别、肿瘤所在部位、肿瘤的Borrmann分型、组织学类型、分化程度、腹膜转移和肝转移无相关性(P>0.05)。结论HPAmRNA阳性表达的胃癌有较高的侵袭转移性,HPA可能是胃癌侵袭转移中的一个重要酶,可能与胃癌的淋巴结转移有关。更多还原

【Abstract】 Objective To explore the relationship between heparanase mRNA expression and clinicopathological parameters in human gastric cancer. Methods RT PCR was used to detect the expression of heparanase mRNA in 43 human gastric carcinomas and 10 adjacent normal gastric tissues. Results Heparanase mRNA was expressed in 29 of the 43 cases of gastric cancer with a positive rate of 67.4%,which was significantly higher than that in adjacent normal gastric tissues ( P =0.013). The expression level was higher in late stage tumors (stage Ⅲ and Ⅳ) than in early stage tumors (stage Ⅰ and Ⅱ) ( P =0.001), in tumors with invasion to serosa than those without serosal invasion ( P =0.009), in tumors with lymph node metastasis than those without lymph node metastasis ( P =0.018), and in large sized tumors than in small sized ones ( P =0.009). The expression was not correlated with patients′ age, sex, tumor location, histologic types, differentiation, peritoneal dissemination and liver metastasis ( P >0.05). Conclusion Heparanase might play an important role in the development of invasion and metastasis of gastric cancer.更多还原